1WDN
GLUTAMINE-BINDING PROTEIN
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1995-01-25 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.700, 91.000, 34.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.940 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.30000 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.450 |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.900 * | 1.940 * |
Rmerge | 0.082 | |
Total number of observations | 65773 * | |
Number of reflections | 15110 | |
Completeness [%] | 91.1 | 82.5 * |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 * | 18 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | PEG4000 | 15 (%(w/v)) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | sodium acetate | 0.1 (M) |