1WA4
Crystal structure of the M131F L135A EvaD double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-14 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 51.632, 79.101, 48.777 |
Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
Resolution | 48.220 - 2.100 |
R-factor | 0.18 |
Rwork | 0.176 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ofn |
RMSD bond length | 0.020 |
RMSD bond angle | 1.997 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 2.160 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.070 | 0.180 |
Number of reflections | 9756 | |
<I/σ(I)> | 7.2 | 5.1 |
Completeness [%] | 98.6 | 95.3 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 100MM TRIS, PH8, 150MM NACL, 25%W/V PEG8000, pH 8.00 |