1VY7
Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site.
これはPDB形式変換不可エントリーです。
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 207.560, 444.230, 613.030 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 150.383 - 2.800 |
| R因子 | 0.236 |
| Rwork | 0.234 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.991 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 222.115 | 222.115 | 2.870 |
| 分解能 [Å] (高) | 2.800 | 12.520 | 2.800 |
| Rmerge_l_obs | 0.184 | 0.013 | 0.013 |
| 独立反射数 | 1345662 | 15056 | 98742 |
| <I/σ(I)> | 6.04 | 16.98 | 1.02 |
| 完全性 [%] | 98.0 | 92.4 | 97.8 |
| 冗長性 | 3.79 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K |
