1VWK
STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2
Experimental procedure
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 95.010, 105.420, 47.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.450 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.24000 |
RMSD bond length | 0.019 |
RMSD bond angle | 25.300 * |
Data reduction software | BIOTEX ((MSC)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.330 * |
Rmerge | 0.079 |
Total number of observations | 82155 * |
Number of reflections | 41763 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 * | 20 * | Pahler, A., (1987) J. Biol. Chem., 262, 13933. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium salfate | 30-32 (%sat) | |
2 | 1 | reservoir | potassium acetate | 0.1 (M) | pH4.0 |
3 | 1 | drop | protein | 15 (mg/ml) | |
4 | 1 | drop | ammonium salfate | 15-16 (%sat) | |
5 | 1 | drop | potassium acetate | 0.05 (M) |