1VWK

STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 2 2 2
Unit cell lengths	95.010, 105.420, 47.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.450
R-factor	0.205
Rwork	0.205
R-free	0.24000
RMSD bond length	0.019
RMSD bond angle	25.300 *
Data reduction software	BIOTEX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.330 *
Rmerge	0.079
Total number of observations	82155 *
Number of reflections	41763
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	30-32 (%sat)
2	1	reservoir	potassium acetate	0.1 (M)	pH4.0
3	1	drop	protein	15 (mg/ml)
4	1	drop	ammonium salfate	15-16 (%sat)
5	1	drop	potassium acetate	0.05 (M)