1VQI

GENE V PROTEIN MUTANT WITH ILE 47 REPLACED BY VAL 47 (I47V)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	75.990, 28.090, 42.630
Unit cell angles	90.00, 103.51, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.205
Rwork	0.205
RMSD bond length	0.012
RMSD bond angle	2.570
Data reduction software	MAR (600)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.041
Total number of observations	47811 *
Number of reflections	7906
Completeness [%]	96.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mM)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	PEG3400	15 (%(w/v))
4	1	reservoir	PEG3400	12 (%)