1VQF

GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY VAL 47 (V35I, I47V)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	76.360, 28.120, 42.500
Unit cell angles	90.00, 103.65, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.21
Rwork	0.210
RMSD bond length	0.013
RMSD bond angle	2.560
Data reduction software	MAR (600)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.042
Total number of observations	25763 *
Number of reflections	7473
Completeness [%]	91.4
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mM)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	PEG3400	15 (%(w/v))
4	1	reservoir	PEG3400	12 (%)