1VDC

STRUCTURE OF NADPH DEPENDENT THIOREDOXIN REDUCTASE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG
Synchrotron site	EMBL/DESY, HAMBURG
Temperature [K]	175
Detector technology	IMAGE PLATE
Collection date	1995-07
Detector	RIGAKU
Spacegroup name	P 41 21 2
格子定数 [Å]	89.974, 89.974, 175.626
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	20.000 - 2.500
R因子	0.188
Rwork	0.188
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1trb
結合長の平均二乗偏差(RMSD) [Å]	0.012
結合角の平均二乗偏差(RMSD) [度]	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	2.540
分解能 [Å] (高)	2.500	2.500
Rmerge_l_obs	0.085	0.487
Total number of observations	139995 *
独立反射数	20556 *
<I/σ(I)>	7.7
完全性 [%]	100.0	100
冗長性	6.81

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *			7% ISOPROPANOL (OR 4% MPD) AND 1.8 M AMMONIUM SULFATE

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	isopropanol	7 (%(v/v))	or 4 % MPD
2	1	reservoir	ammonium sulfate	1.8 (M)