1VBG
Pyruvate Phosphate Dikinase from Maize
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.157, 100.222, 108.406 |
Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
Resolution | 46.030 - 2.300 |
R-factor | 0.215 |
Rwork | 0.209 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dik |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.290 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.054 | 0.301 |
Number of reflections | 51096 | |
<I/σ(I)> | 11.5 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8000, magnesium sulfate, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |