1UYN
Translocator domain of autotransporter NalP from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Collection date | 2002-05-15 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.970, 57.970, 346.435 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.28500 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.064 | 0.191 |
| Total number of observations | 97916 * | |
| Number of reflections | 10715 | |
| <I/σ(I)> | 24 | 9.2 |
| Completeness [%] | 98.5 | 88.7 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 28 * | 200 MM LI2SO4, 100 MM NA CIT BUFFER, PH 4.0, 9% PEG 1000. PROTEIN WAS CRYSTALLISED IN THE PRESENCE OF 0.06% N-DECYLPENTAOXYETHYLENE AND 0.5% HEPTYL-B-GLUCOPYRANOSIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 0.06 (%(w/v)) | ||
| 4 | 1 | drop | heptyl-beta-glucopyranoside | 0.5 (%(w/v)) | |
| 5 | 1 | reservoir | PEG1000 | 9 (%(w/v)) | |
| 6 | 1 | reservoir | lithium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.0 |
