1UWR
Structure of beta-glycosidase from Sulfolobus solfataricus in complex with 2-deoxy-2-fluoro-galactose
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 168.059, 168.059, 95.773 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 141.420 - 2.140 |
| R因子 | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwq |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.357 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.230 |
| 分解能 [Å] (高) | 2.150 | 2.150 |
| Rmerge_l_obs | 0.117 | 0.455 |
| 独立反射数 | 85809 | |
| <I/σ(I)> | 10.1 | 2.82 |
| 完全性 [%] | 99.9 | 100 |
| 冗長性 | 5.89 | 5.86 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 4.6 | 11-14% PEG 4K 0.1 M NA ACETATE, 0.2 M AMMONIUM ACETATE. CRYO - 25% ETHYLENE GLYCOL, 10-13 MG/ML PROTEIN, pH 4.6 |
