1UTC
Clathrin terminal domain complexed with TLPWDLWTT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.301, 61.301, 420.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 * - 2.300 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c9i |
RMSD bond length | 0.016 |
RMSD bond angle | 1.600 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 * | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.076 * | 0.329 * |
Total number of observations | 495661 * | |
Number of reflections | 37078 * | |
<I/σ(I)> | 9.3131 | 2.02 |
Completeness [%] | 99.4 | 97.2 * |
Redundancy | 5.8 * | 4.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | VAPOUR DIFFUSION OVER A RESERVOIR CONTAINING 20% PEG8000, 200 MM MGCL2, 4 MM DTT,100 MM CAPS PH 9, DRIED UP DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
2 | 1 | reservoir | 200 (mM) | ||
3 | 1 | reservoir | dithiothreitol | 4 (mM) | |
4 | 1 | reservoir | CAPS | 100 (mM) | pH9. |