1UNA

UNASSEMBLED VIRUS COAT PROTEIN DIMER, BACTERIOPHAGE RNA-BINDING DIMER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1993-12-03
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
格子定数 [Å]	59.300, 60.500, 67.100
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	6.000 - 2.800
Rwork	0.204
R-free	0.28400
Structure solution method	MOL. REPLACEMENT
Starting model (for MR)	MS2 COAT PROTEIN DIMER (1MS2)
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.031
Data reduction software	XENGEN ((HOWARD)
Phasing software	X-PLOR
Refinement software	PROLSQ

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	4.790 *	3.000
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs	0.074 *	0.123 *
Total number of observations	32352 *
独立反射数	6248 *	1157 *
<I/σ(I)>	12.9	5
完全性 [%]	94.0	81
冗長性	3	2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.2 *	13 *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	GA dimers	3 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop		50 (mM)
4	1	reservoir	Tris-maleate	0.1 (M)
5	1	reservoir	PEG1000