1UAA
E. COLI REP HELICASE/DNA COMPLEX
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 141.800, 141.800, 284.800 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 15.000 - 3.000 |
| R因子 | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.32800 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.019 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.918 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 30.000 |
| 分解能 [Å] (高) | 3.000 |
| Rmerge_l_obs | 0.053 |
| Total number of observations | 434546 * |
| 独立反射数 | 57472 |
| 完全性 [%] | 97.3 |
| 冗長性 | 7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | pH 8.00, VAPOR DIFFUSION, temperature 293.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | TRIS-HCL | ||
| 4 | 1 | 1 | EDTA | ||
| 5 | 1 | 1 | GLYCEROL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | GLYCEROL |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | drop | PEG8000 | 2.5 (%) | |
| 7 | 1 | reservoir | glycerol | 2 (%(v/v)) |
