1U1Z
The Structure of (3R)-hydroxyacyl-ACP dehydratase (FabZ)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97943 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.031, 100.897, 177.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.350 - 2.500 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.25800 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNX (2000.1) |
Refinement software | CNX (2000.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 2.590 |
High resolution limit [Å] | 2.490 | 2.500 |
Number of reflections | 108745 | |
<I/σ(I)> | 17.1 | 3 |
Completeness [%] | 97.0 | 90.9 |
Redundancy | 5.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Ammonium Sulphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |