1U1G
Structure of E. coli uridine phosphorylase complexed to 5-(m-(benzyloxy)benzyl)barbituric acid (BBBA)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 92.677, 127.124, 143.355 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 49.390 - 1.950 |
| R因子 | 0.20121 |
| Rwork | 0.200 |
| R-free | 0.22148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.897 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 49.390 | 2.020 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| 独立反射数 | 118727 | |
| 完全性 [%] | 94.9 | 71.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | PEG 4000, MES, GLYCEROL, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
