1U1E
Structure of e. coli uridine phosphorylase complexed to 5(phenylseleno)acyclouridine (PSAU)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 91.076, 125.690, 140.899 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 48.800 - 2.001 |
| R因子 | 0.21351 |
| Rwork | 0.211 |
| R-free | 0.23452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| 独立反射数 | 108334 | |
| 完全性 [%] | 99.1 | 94.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | PEG 4000, MES, GLYCEROL, PH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
