1U1C

Structure of E. coli uridine phosphorylase complexed to 5-benzylacyclouridine (BAU)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
格子定数 [Å]	91.369, 125.965, 141.334
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	38.070 - 2.200
R因子	0.21878
Rwork	0.215
R-free	0.24858
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	0.883
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	38.070	2.280
分解能 [Å] (高)	2.200	2.200
Rmerge_l_obs	0.054	0.331
独立反射数	83127
完全性 [%]	99.9	99.9
冗長性	6.5	6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.2	295	PEG 4000, MES, GLYCEROL, PH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K