1TVD
VARIABLE DOMAIN OF T CELL RECEPTOR DELTA CHAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 278 |
| Detector technology | CCD |
| Collection date | 1996-06 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 69.940, 49.080, 61.670 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 6.000 - 1.900 |
| R因子 | 0.161 |
| Rwork | 0.161 |
| R-free | 0.22400 |
| Structure solution method | MIRAS |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.600 * |
| Data reduction software | MADNES |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 99.000 | 2.000 |
| 分解能 [Å] (高) | 1.900 | 1.900 |
| Rmerge_l_obs | 0.093 | 0.166 |
| Total number of observations | 44342 * | |
| 独立反射数 | 13905 * | |
| <I/σ(I)> | 10 | 3 |
| 完全性 [%] | 83.3 | 53.7 |
| 冗長性 | 3 | 2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 7 * | Lebedeva, M.I., (1996) Protein Sci., 5, 2638. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.9 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH7.0 |
