1TGV

Structure of E. coli Uridine Phosphorylase complexed with 5-Fluorouridine and sulfate

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-10
Detector	ADSC QUANTUM 4
Spacegroup name	H 3
格子定数 [Å]	149.972, 149.972, 49.566
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	20.000 - 2.200
Rwork	0.175
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	1.300
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall
分解能 [Å] (低)	30.000
分解能 [Å] (高)	2.200
独立反射数	19412
<I/σ(I)>	14.4
完全性 [%]	91.9
冗長性	2.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	PEG 4000, MES, Glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K