1TG9

Effect of Shuttle Location and pH Environment on H+ Transfer in Human Carbonic Anhydrase II

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
格子定数 [Å]	42.643, 41.617, 72.905
格子定数 [度]	90.00, 104.56, 90.00

精密化法

残基	20.000 - 1.900
Rwork	0.168
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cba
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	1.345
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	1.930
分解能 [Å] (高)	1.900	1.900
独立反射数	18130
完全性 [%]		90.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	50 mM Tris-Cl pH7.8 , 2.5 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K