1TFK

Ribonuclease from Escherichia coli complexed with its inhibtor protein

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	95
Detector technology	CCD
Collection date	2003-03-27
Detector	MARRESEARCH
Wavelength(s)	0.97919, 0.97937, 0.98200
Spacegroup name	P 41 21 2
格子定数 [Å]	58.503, 58.503, 149.392
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	23.100 - 2.100
R因子	0.228
Rwork	0.225
R-free	0.25100
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	1.100
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	23.100	2.200
分解能 [Å] (高)	2.100	2.100
独立反射数	15793
完全性 [%]	99.4	99.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, DTT, magnesium acetate, MES, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K