1TF5

Crystal structure of SecA in an open conformation from Bacillus Subtilis

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Wavelength(s)	0.97903
Spacegroup name	C 1 2 1
格子定数 [Å]	149.232, 107.189, 72.054
格子定数 [度]	90.00, 94.96, 90.00

精密化法

残基	50.000 - 2.180
R因子	0.241
Rwork	0.241
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m6n
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.260
分解能 [Å] (高)	2.180	2.180
独立反射数	58197
<I/σ(I)>	28.37
完全性 [%]	98.8	97.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES, 250mM Magnesium Acetate, 7-12% PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K