1TES
OXYGEN BINDING MUSCLE PROTEIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06-06 |
| Detector | RIGAKU |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.622, 91.622, 45.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 1.700 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 21.700 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.600 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.062 |
| Number of reflections | 18453 |
| Completeness [%] | 75.1 |
| Redundancy | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 20 * | pH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.2-2.6 (M) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | EDTA | 1 (mM) | |
| 4 | 1 | drop | protein | 5 (mg/ml) |
