1TDK
L-amino acid oxidase from Agkistrodon halys in complex with suicide substrate L-vinylglycine
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Spacegroup name | I 21 3 |
| 格子定数 [Å] | 169.360, 169.360, 169.360 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 45.260 - 2.700 |
| R因子 | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1reo |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.750 |
| 分解能 [Å] (高) | 2.650 | 2.650 |
| Rmerge_l_obs | 0.051 | 0.447 |
| 独立反射数 | 176710 | |
| <I/σ(I)> | 1.1 | |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 7.03 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
