1T7A
Crystal structure of mutant Lys8Asp of scorpion alpha-like neurotoxin BmK M1 from Buthus martensii Karsch
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 283 |
Detector technology | CCD |
Collection date | 2000-11-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
格子定数 [Å] | 47.452, 44.343, 25.491 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 30.000 - 1.500 |
R因子 | 0.165 |
Rwork | 0.165 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sn1 |
結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
結合角の平均二乗偏差(RMSD) [度] | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 30.000 | 1.550 |
分解能 [Å] (高) | 1.500 | 1.500 |
Rmerge_l_obs | 0.052 | 0.175 |
独立反射数 | 8946 | |
<I/σ(I)> | 28.3 | 8.7 |
完全性 [%] | 98.1 | 93.3 |
冗長性 | 4.6 | 4.3 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | sodium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |