1T6Q
Nickel Superoxide Dismutase (NiSOD) CN-treated Apo Structure
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.549784 |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 60.041, 112.491, 111.298 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 47.830 - 2.050 |
| R因子 | 0.217 |
| Rwork | 0.217 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t6i |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 100.000 | 2.120 |
| 分解能 [Å] (高) | 2.050 | 2.050 |
| 独立反射数 | 23036 | |
| <I/σ(I)> | 24.9 | 5.1 |
| 完全性 [%] | 96.4 | 85.4 |
| 冗長性 | 7.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | MPEG 5000, Hepes, methanol, calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
