1T1L
Crystal structure of the long-chain fatty acid transporter FadL
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07, 0.97 |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 122.020, 122.020, 164.736 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 11.990 - 2.800 |
| R因子 | 0.306 |
| Rwork | 0.298 |
| R-free | 0.33100 |
| Structure solution method | MIR |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 49.030 | 2.800 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| 独立反射数 | 36854 | |
| 完全性 [%] | 93.6 | |
| 冗長性 | 3.3 | 2.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | Ammonium Formate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | Ammonium Formate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
