1T16
Crystal structure of the bacterial fatty acid transporter FadL from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.380, 68.664, 106.024 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.262 |
| Rwork | 0.257 |
| R-free | 0.30100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.120 |
| High resolution limit [Å] | 2.490 |
| Number of reflections | 46492 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | PEG 4000, Copper(II)chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | PEG 4000, Copper(II)chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
