1STR
STREPTAVIDIN DIMERIZED BY DISULFIDE-BONDED PEPTIDE AC-CHPQNT-NH2 DIMER
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 96.740, 106.040, 48.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.800 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.23300 |
RMSD bond length | 0.018 |
RMSD bond angle | 27.640 * |
Data reduction software | SADIE |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.740 |
Rmerge | 0.074 |
Total number of observations | 60758 * |
Number of reflections | 19758 |
Completeness [%] | 74.0 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 30-32 (%sat) | |
2 | 1 | reservoir | potassium acetate | 0.1 (M) |