1STC
CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT IN COMPLEX WITH STAUROSPORINE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.990, 72.820, 76.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.33300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cdk |
RMSD bond length | 0.012 |
RMSD bond angle | 1.547 |
Data reduction software | MOSFLM |
Data scaling software | Agrovata |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.073 * | 0.246 * |
Total number of observations | 64256 * | |
Number of reflections | 16993 | |
<I/σ(I)> | 3 | |
Completeness [%] | 92.2 | 92.3 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.4 * | 5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MES-Bis-Tris | 25 (mM) | |
2 | 1 | drop | octanoyl-N-methylglucamide | 1.5 (mM) | |
3 | 1 | reservoir | methanol | 15 (%) |