1SO8

Abeta-bound human ABAD structure [also known as 3-hydroxyacyl-CoA dehydrogenase type II (Type II HADH), Endoplasmic reticulum-associated amyloid beta-peptide binding protein (ERAB)]

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Spacegroup name	P 4 3 2
格子定数 [Å]	130.000, 130.000, 130.000
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.830 - 2.300
R因子	0.231
Rwork	0.231
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.051	0.152
独立反射数	17049
<I/σ(I)>	57.7	13
完全性 [%]	99.8	99.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6	295	Abeta, MES, NaCl, DTT, NAD, benzamidine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K