1SLQ
Crystal structure of the trimeric state of the rhesus rotavirus VP4 membrane interaction domain, VP5CT
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.916 |
| Spacegroup name | P 42 2 2 |
| 格子定数 [Å] | 244.843, 244.843, 104.778 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 3.200 |
| R因子 | 0.308 * |
| Rwork | 0.308 |
| R-free | 0.33800 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.930 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 3.270 |
| 分解能 [Å] (高) | 3.200 | 3.200 |
| Rmerge_l_obs | 0.057 | 0.120 |
| 独立反射数 | 51973 | 3446 * |
| <I/σ(I)> | 23.6 | 8.8 |
| 完全性 [%] | 99.8 | 99.8 |
| 冗長性 | 8.4 | 7.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 23 * | ammonium sulfate, MPD, Pipes, Tris, EDTA, sodium azide, benzamidine, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 23 * | ammonium sulfate, MPD, Pipes, Tris, EDTA, sodium azide, benzamidine, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | sodium azide | 0.02 (%) | |
| 5 | 1 | drop | benzamidine | 0.1 (mM) | |
| 6 | 1 | reservoir | ethanol | 6 (%) | |
| 7 | 1 | reservoir | Tris | 50 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 1 (mM) |
