1SL9
Obelin from Obelia longissima
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 83.021, 54.341, 52.442 |
| 格子定数 [度] | 90.00, 112.15, 90.00 |
精密化法
| 残基 | 10.000 - 1.170 |
| R因子 | 0.1628 |
| Rwork | 0.157 |
| R-free | 0.19550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el4 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.032 |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.210 |
| 分解能 [Å] (高) | 1.170 | 1.170 |
| Rmerge_l_obs | 0.045 | 0.249 |
| 独立反射数 | 61453 | |
| 完全性 [%] | 84.7 | 65.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 8000, potassium phosphate, hexaminecobaltic chloride , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
