1S6B
X-ray Crystal Structure of a Complex Formed Between Two Homologous Isoforms of Phospholipase A2 from Naja naja sagittifera: Principle of Molecular Association and Inactivation
Replaces: 1LFJExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2001-09-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 41 |
| Unit cell lengths | 64.537, 64.537, 57.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.212 |
| Rwork | 0.206 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1psh |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 * | 0.329 * |
| Total number of observations | 500751 * | |
| Number of reflections | 27946 | |
| <I/σ(I)> | 26 | 3.02 |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | calcium chloride, tris, pH 6.00, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 10 (mM) | |
| 2 | 1 | drop | calcium acetate | 20 (mM) | |
| 3 | 1 | drop | protein | 15 (mg/ml) | |
| 4 | 1 | reservoir | ethanol | 25 (%) |
