1RTR
Crystal Structure of S. Aureus Farnesyl Pyrophosphate Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 97 |
Detector technology | CCD |
Collection date | 2002-11-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 150.201, 150.201, 163.184 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.22662 |
Rwork | 0.222 |
R-free | 0.26573 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.211 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 38076 | |
<I/σ(I)> | 16 | |
Completeness [%] | 97.0 | 0.93 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Nanoliter Sitting Drop Vapour Diffusion | 5 | 298 | PEG 1000, Phosphate, pH 5, Nanoliter Sitting Drop Vapour Diffusion, temperature 298K |