1RKS
E. COLI RIBOKINASE IN COMPLEX WITH D-RIBOSE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 95.210, 95.210, 154.460 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 15.000 - 2.400 |
| R因子 | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.28800 |
| Structure solution method | OTHER |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 29.000 | 2.440 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| Rmerge_l_obs | 0.069 | 0.354 |
| Total number of observations | 134333 * | |
| 独立反射数 | 16814 | |
| <I/σ(I)> | 30.2 | 5.8 |
| 完全性 [%] | 99.9 | 99.8 |
| 冗長性 | 10.5 | 7.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 20 * | 5 MM RIBOSE, 10 MM MGCL2 IN THE DROP, 0.1 M TRIS-HCL BUFFER, PH 8.4 WITH 2.1-2.4 M NH4H2PO4 AS PRECIPITANT, VAPOR DIFFUSION, HANGING DROP CRYO-SOLUTION: MOTHER LIQUOR CONTAINING 20% GLYCEROL. SOAK-TIME: 2-3 MINUTES. |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | ribose | 5 (mM) | |
| 2 | 1 | reservoir | ADP | 10 (mM) | |
| 3 | 1 | reservoir | 0.05 (mM) | ||
| 4 | 1 | reservoir | 15 (mM) | ||
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 6 | 1 | reservoir | 0.25 (M) | ||
| 7 | 1 | reservoir | MPD | 20 (%(v/v)) | |
| 8 | 1 | reservoir | PEG4000 | 8.8-15 (%(w/v)) | |
| 9 | 1 | reservoir | ribokinase | 3-3.5 (mg/ml) |
