1RKD
E. COLI RIBOKINASE COMPLEXED WITH RIBOSE AND ADP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 95.400, 95.400, 154.870 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 28.000 - 1.840 |
| R因子 | 0.221 |
| Rwork | 0.220 |
| R-free | 0.25800 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 1.870 |
| 分解能 [Å] (高) | 1.840 | 1.840 |
| Rmerge_l_obs | 0.055 | 0.239 |
| Total number of observations | 279852 * | |
| 独立反射数 | 35015 | |
| <I/σ(I)> | 30.2 | 7.4 |
| 完全性 [%] | 95.5 | 97.8 |
| 冗長性 | 7.5 | 7.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8.4 | Sigrell, J.A., (1997) Protein Sci., 6, 2474. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | ribokinase | 5.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | ribose | 0.5 (mM) | |
| 5 | 1 | drop | Li AMP-PNP | 10 (mM) | |
| 6 | 1 | drop | 10 (mM) | ||
| 7 | 1 | drop | 1.05-1.2 (M) | ||
| 8 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 9 | 1 | reservoir | ammonium phosphate |
