1RKA
THE APO FORM OF E. COLI RIBOKINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.970, 61.970, 127.834 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.210 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RKD: RESIDUES 4-9 44-93 123-241 AND 254-309. |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.450 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 | 0.235 |
| Total number of observations | 62741 * | |
| Number of reflections | 12644 | |
| <I/σ(I)> | 20.6 | 6.7 |
| Completeness [%] | 96.4 | 87.5 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.8 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ribokinase | 11 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | reservoir | Na acetate | 0.1 (M) | |
| 5 | 1 | reservoir | NH4 acetate | 0.2 (M) | |
| 6 | 1 | reservoir | mPEG2000 | 22-33 (%) |
