1RHZ
The structure of a protein conducting channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9799 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.746, 149.361, 79.475 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.990 - 3.500 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.33400 * |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.080 * | 0.670 * |
| Number of reflections | 14439 * | 1291 * |
| <I/σ(I)> | 30.9 | 2.74 |
| Completeness [%] | 98.4 * | 89.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 4 * | Peg 400, Glycine buffer, glycerol, sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 50-55 (%) | |
| 3 | 1 | reservoir | glycine | 50 (mM) | pH9.0-9.5 |
