1R7L
2.0 A Crystal Structure of a Phage Protein from Bacillus cereus ATCC 14579
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9798, 0.9465 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 37.002, 66.338, 53.966 |
| 格子定数 [度] | 90.00, 95.29, 90.00 |
精密化法
| 残基 | 24.900 - 2.000 |
| R因子 | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.098 | 0.518 |
| 独立反射数 | 17557 | |
| <I/σ(I)> | 22.3 | 2.25 |
| 完全性 [%] | 99.1 | 95.6 |
| 冗長性 | 8.12 | 7.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 20% PEG 8000, 0.1M MES, 0.2M Ca(OAc)2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
