1R10

Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) with ATP, I4122 space group

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	I 41 2 2
格子定数 [Å]	141.200, 141.200, 279.460
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	30.000 - 3.000
R因子	0.232
Rwork	0.228
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.012
結合角の平均二乗偏差(RMSD) [度]	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	49.700	3.110
分解能 [Å] (高)	3.000	3.000
Rmerge_l_obs	0.073 *	0.980 *
独立反射数	28573
完全性 [%]	100.0	100
冗長性	8.2 *	8.3 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	4, 20 *	sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	sodium acetate	3.5-4.0 (M)