1QZH
Crystal structure of Pot1 (protection of telomere)- ssDNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2003-02-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0688 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.614, 76.329, 140.130 |
Unit cell angles | 90.00, 115.21, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.430 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.062 * | |
Total number of observations | 132615 * | |
Number of reflections | 43793 | |
Completeness [%] | 89.4 * | 78.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 16 * | PEG4000, TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | TRIS | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | PEG4000 | ||
5 | 1 | 2 | TRIS | ||
6 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
2 | 1 | reservoir | PEG4000 | 27-30 (%) | |
3 | 1 | reservoir | dithiothreitol | 5 (mM) |