1QLE
CRYO-STRUCTURE OF THE PARACOCCUS DENITRIFICANS FOUR-SUBUNIT CYTOCHROME C OXIDASE IN THE COMPLETELY OXIDIZED STATE COMPLEXED WITH AN ANTIBODY FV FRAGMENT
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 |
| 格子定数 [Å] | 205.200, 205.200, 81.100 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 6.000 - 3.000 |
| R因子 | 0.235 |
| Rwork | 0.235 |
| R-free | 0.30900 |
| Structure solution method | OTHER |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 23.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 20.000 |
| 分解能 [Å] (高) | 3.000 |
| Rmerge_l_obs | 0.115 |
| Total number of observations | 165650 * |
| 独立反射数 | 64653 |
| <I/σ(I)> | 9.6 |
| 完全性 [%] | 95.2 |
| 冗長性 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, sitting drop * | 8 | 14 * | pH 8.00 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | ammonium acetate | 400 (mM) | |
| 2 | 1 | reservoir | 7 (%) | ||
| 3 | 1 | reservoir | mPEG2000 | 10-14 (%) |
