Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 68.350, 71.980, 131.000 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 15.000 - 2.000 |
R因子 | 0.21 * |
Rwork | 0.210 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 14-3-3 ZETA |
結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
結合角の平均二乗偏差(RMSD) [度] | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 15.000 | 2.090 |
分解能 [Å] (高) | 2.000 | 2.000 |
Rmerge_l_obs | 0.059 | 0.320 |
独立反射数 | 37059 | |
<I/σ(I)> | 13.3 | 2.1 |
完全性 [%] | 83.5 | 41.6 |
冗長性 | 2 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 6.2 | 4 * | used to seeding * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | reservoir | MES | 100 (mM) | |
2 | 1 | reservoir | 20 (mM) | ||
3 | 1 | reservoir | isopropanol | 15-17 (%) | |
4 | 1 | reservoir | PEG4000 | 12 (%) |