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CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-15
Detector	MARRESEARCH
Spacegroup name	H 3 2
格子定数 [Å]	82.300, 82.300, 208.100
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	35.000 - 2.100
R因子	0.233 *
Rwork	0.233
R-free	0.28400
Structure solution method	MIR
結合長の平均二乗偏差(RMSD) [Å]	0.021
結合角の平均二乗偏差(RMSD) [度]	0.041
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	35.000	2.200
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.076 *	0.320 *
独立反射数	16165
<I/σ(I)>	11	2.7
完全性 [%]	99.0	99
冗長性	3.6	3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	8.5 *		30 % (V/V) 2-PROPANOL, 20 % (V/V) GLYCEROL, 0.2 M CACL2, 0.1 M NA-ACETATE PH 4.6

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	2-propanol	30 (%(v/v))
2	1	reservoir	glycerol	20 (%(v/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	sodium acetate	0.1 (M)
5	1	drop	protain	20 (mg/ml)
6	1	drop		0.6 (%(v/v))
7	1	drop	Tris-HCl	20 (mM)