1QH5
HUMAN GLYOXALASE II WITH S-(N-HYDROXY-N-BROMOPHENYLCARBAMOYL)GLUTATHIONE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-01 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.550, 73.800, 164.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.115 | 0.340 |
| Total number of observations | 437671 * | |
| Number of reflections | 84815 * | |
| <I/σ(I)> | 13 | 4 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.1 * | EQUILABRATION AGAINST 4-20% W/V PEG 2000 MONOMETHYL ETHER, pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | MOPS | 10 (mM) | |
| 3 | 1 | drop | PEG400 | 25 (%) | |
| 4 | 1 | reservoir | mPEG2000 | 4-20 (%) | |
| 5 | 1 | reservoir | sodium imidazole | 0.1 (M) | |
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) |
