1QB2

CRYSTAL STRUCTURE OF THE CONSERVED SUBDOMAIN OF HUMAN PROTEIN SRP54M AT 2.1A RESOLUTION: EVIDENCE FOR THE MECHANISM OF SIGNAL PEPTIDE BINDING

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09-13
Detector	BRANDEIS
Spacegroup name	P 21 21 21
格子定数 [Å]	28.910, 61.340, 129.220
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.000 - 2.100
Rwork	0.245
R-free	0.31800
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.065
Data reduction software	MADNESS
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.843)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.140
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.057	0.296
Total number of observations	165944 *
独立反射数	25000
完全性 [%]	99.0	94
冗長性	3.5	2.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, sitting drop *	4.6	4 *	30% PEG 2K, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	Vapor diffusion, sitting drop *	4.6	4 *	30% PEG 2K, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	Vapor diffusion, sitting drop *	4.6	4 *	30% PEG 2K, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	Vapor diffusion, sitting drop *	4.6	4 *	30% PEG 2K, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	25 (mg/ml)
2	1	drop		0.15 (M)
3	1	drop	Tris-HCl	50 (mM)	pH8.0
4	1	drop	PEG2000 MME	25 (%(w/v))
5	1	drop	ammonium sulfate	0.2 (M)
6	1	drop	sodium acetate	0.1 (M)	pH4.6
7	1	reservoir	glycerol	15 (%(v/v))