1QAM
THE STRUCTURE OF THE RRNA METHYLTRANSFERASE ERMC': IMPLICATIONS FOR THE REACTION MECHANISM
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 81.380, 81.380, 122.220 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.200 |
| R因子 | 0.221 * |
| Rwork | 0.221 |
| R-free | 0.25100 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.280 |
| 分解能 [Å] (高) | 2.200 | 2.200 |
| Rmerge_l_obs | 0.054 | 0.308 |
| Total number of observations | 72558 * | |
| 独立反射数 | 20680 | |
| <I/σ(I)> | 17 | |
| 完全性 [%] | 96.0 | 94.4 |
| 冗長性 | 3.5 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 8000, AMMONIUM ACETATE, PH 7.8 AT 298 K, VAPOR DIFFUSION, HANGING DROP |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | Tes | 100 (mM) | |
| 7 | 1 | reservoir | ammonium acetate | 500 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 15 (%(w/v)) |
