1PZD
Structural Identification of a conserved appendage domain in the carboxyl-terminus of the COPI gamma-subunit.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.570, 89.560, 98.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.190 - 2.310 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 25.100 * |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 19621 | |
<I/σ(I)> | 6.3 | |
Completeness [%] | 89.7 | 87.5 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | 100mM sodium citrate and 1.5-1.7M lithium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 18K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | 100mM sodium citrate and 1.5-1.7M lithium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 18K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH7.5 |
3 | 1 | reservoir | 1.4-1.7 (M) | ||
4 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |