1PRY
Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.74 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 43.967, 62.082, 103.363 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 40.060 * - 1.970 |
R因子 | 0.228 * |
Rwork | 0.204 |
R-free | 0.27200 * |
Structure solution method | Single Anomalous Scattering |
結合長の平均二乗偏差(RMSD) [Å] | 0.006 * |
結合角の平均二乗偏差(RMSD) [度] | 1.300 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 40.460 | 2.050 |
分解能 [Å] (高) | 1.970 | 1.970 |
Rmerge_l_obs | 0.052 * | |
独立反射数 | 18879 | |
<I/σ(I)> | 19.4 | |
完全性 [%] | 82.3 * | 33.9 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Batch method * | 8.5 | 18 * | PEG 6000, ammonium phosphate, pH 8.5, Microbatch, temperature 291K |
1 | Batch method * | 8.5 | 18 * | PEG 6000, ammonium phosphate, pH 8.5, Microbatch, temperature 291K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | 1 | PEG8000 | 10 (%) | |
2 | 1 | 1 | calcium acetate | 200 (mM) | |
3 | 1 | 1 | imidazole | 100 (mM) | pH8.0 |
4 | 1 | 1 | PEG6000 | 12 (%) | |
5 | 1 | 1 | di-ammonium hydrogen phosphate | 100 (mM) | |
6 | 1 | 1 | Tris-HCl | 100 (mM) | pH8.5 |